Tuning Monoclonal Antibody Galactosylation using Raman Spectroscopy

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bd-academy

Tuning Monoclonal Antibody Galactosylation using Raman Spectroscopy-Controlled Lactic Acid Feeding

A key aspect of large-scale production of biotherapeutics is a well-designed and consistently-executed upstream cell culture process. Process Analytical Technology (PAT) tools provide enhanced monitoring and control capabilities to support consistent process execution, and also have potential to aid in maintenance of product quality at desired levels.

One such tool, Raman spectroscopy, has matured as a useful technique to achieve real-time monitoring and control of key cell culture process attributes. We developed a Raman spectroscopy-based nutrient control strategy to enable dual control of lactate and glucose levels for a fed-batch CHO cell culture process for monoclonal antibody (mAb) production.

To achieve this, PLS-based chemometric models for real-time prediction of glucose and lactate concentrations were developed and deployed in feedback control loops. In particular, feeding of lactic acid post-metabolic shift was investigated based on previous work that has shown the impact of lactate levels on ammonium as well as mAb product quality.

Three feeding strategies were assessed for impact on cell metabolism, productivity, and product quality: bolus-fed glucose, glucose control at 4 g/L, or simultaneous glucose control at 4 g/L and lactate control at 2 g/L. The third feeding strategy resulted in a significant reduction in ammonium levels (68%) while increasing mAb galactosylation levels by approximately 50%.

This work demonstrated that when deployed in a cell culture process, Raman spectroscopy is an effective technique for simultaneous control of multiple nutrient feeds, and that lactic acid feeding can have a positive impact on both cell metabolism and mAb product quality. This article is protected by copyright. All rights reserved.

bd-academy
bd-academy

Mouse Paraoxonase 1 (PON1) ELISA Kit

RD-PON1-Mu-96Tests 96 Tests
EUR 677

Mouse Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Mu-48T 48T
EUR 489
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Mouse Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Mu-96T 96T
EUR 635
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Anti-PON1 Antibody (monoclonal, 9D3)

M00516 100ug/vial
EUR 334

Anti-PON1 Antibody (monoclonal, 6B1)

M00516-1 100ug/vial
EUR 334

Anti-PON1 Antibody (monoclonal, 11H2)

M00516-2 100ug/vial
EUR 294

Anti-PON1 Clone KRJ2 (100 µg)

0801014 100 µg
EUR 284
Description: Please contact Gentaur in order to receive the datasheet of the product.

Anti-PON1 Clone KRJ2 (1 mg)

0801097 1 mg
EUR 1689.04
Description: Please contact Gentaur in order to receive the datasheet of the product.

Rabbit Anti-Human PON1 Monoclonal Antibody

CABT-36017RH 100ug
EUR 840

anti-PON1

YF-PA24422 50 ul
EUR 334
Description: Mouse polyclonal to PON1

Mouse Monoclonal Anti-Mouse CD160 mAb, Biotin, (clone CNX46-3), (mouse IgG2bk)

MCD160-B 100 tests
EUR 469

Mouse Monoclonal Anti-Mouse CD160 mAb, FITC, (clone CNX46-3), (mouse IgG2bk)

MCD160-F 50 Tests
EUR 408

Mouse Monoclonal Anti-Mouse CD160 mAb, Purified, (clone CNX46-3), (mouse IgG2bk)

MCD160-M 100 ug
EUR 482

Mouse Monoclonal Anti-Mouse CD160 mAb, PE, (clone CNX46-3), (mouse IgG2bk)

MCD160-PE 50 tests
EUR 347

Human Paraoxonase 1 (PON1) ELISA Kit

RDR-PON1-Hu-48Tests 48 Tests
EUR 500

Human Paraoxonase 1 (PON1) ELISA Kit

RDR-PON1-Hu-96Tests 96 Tests
EUR 692

Rat Paraoxonase 1 (PON1) ELISA Kit

RDR-PON1-Ra-48Tests 48 Tests
EUR 534

Rat Paraoxonase 1 (PON1) ELISA Kit

RDR-PON1-Ra-96Tests 96 Tests
EUR 742

Human Paraoxonase 1 (PON1) ELISA Kit

RD-PON1-Hu-48Tests 48 Tests
EUR 478

Human Paraoxonase 1 (PON1) ELISA Kit

RD-PON1-Hu-96Tests 96 Tests
EUR 662

Rat Paraoxonase 1 (PON1) ELISA Kit

RD-PON1-Ra-48Tests 48 Tests
EUR 511

Rat Paraoxonase 1 (PON1) ELISA Kit

RD-PON1-Ra-96Tests 96 Tests
EUR 709

Human Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Hu-48T 48T
EUR 479
Description: A sandwich quantitative ELISA assay kit for detection of Human Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Hu-96T 96T
EUR 621
Description: A sandwich quantitative ELISA assay kit for detection of Human Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Ra-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Ra-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Anti-GFP (GF28R) Tag monoclonal antibody

ABP-MAB-GT007 100 ug Ask for price

PON1 recombinant monoclonal antibody

A5406 100ul X 3
EUR 595
Description: A recombinant monoclonal antibody from rabbit against human PON1 for WB, IHC,ELISA

Anti-?-Actin (BA3R) Loading Control Monoclonal Antibody

ABP-MAB-BL002 100 ug Ask for price

Anti-DYKDDDDK (FLAG) Tag (FG4R) Monoclonal Antibody

ABP-MAB-DT006 100 ug Ask for price

Anti-ERK1 (E32) Signal Transduction Monoclonal Antibody

ABP-MAB-ES001 100 ug Ask for price

Anti-ERK1 (E19) Signal Transduction Monoclonal Antibody

ABP-MAB-ES002 100 ug Ask for price

Anti-GAPDH (GA1R) Loading Control Monoclonal Antibody

ABP-MAB-GL001 100 ug Ask for price

Anti-GST Tag (GST.B6 / G2R) Monoclonal Antibody

ABP-MAB-GT003 100 ug Ask for price

Anti-His Tag (HIS.H8 / EH158) Monoclonal Antibody

ABP-MAB-HT001 100 ug Ask for price

Anti-HA Tag (HA.C5 / C10) Monoclonal Antibody

ABP-MAB-HT002 100 ug Ask for price

Anti-Myc Tag (Myc.A7 / M19) Monoclonal Antibody

ABP-MAB-MT005 100 ug Ask for price

Anti-V5 Tag (E10 / V4RR) Monoclonal Antibody

ABP-MAB-VT004 100 ug Ask for price

Anti-PON1 Antibody

PA2253 100ug/vial
EUR 294

Anti-PON1 Antibody

A00516 100ug/vial
EUR 334

Anti-PON1 Antibody

A00516-1 100ug/vial
EUR 294

Anti-PON1 Antibody

A00516-3 100ug/vial
EUR 334

Anti-PON1 antibody

STJ25055 100 µl
EUR 277
Description: The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.

Anti-PON1 antibody

STJ25056 100 µl
EUR 277
Description: The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.

Anti-PON1 antibody

STJ29477 100 µl
EUR 277
Description: The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.

Anti-PON1 antibody

STJ98804 200 µl
EUR 197
Description: Rabbit polyclonal to PON1.

anti- PON1 antibody

FNab06638 100µg
EUR 505.25
Description: Antibody raised against PON1

Anti-PON1 (2H7)

YF-MA14808 100 ug
EUR 363
Description: Mouse monoclonal to PON1

Anti-PON1 antibody

PAab06638 100 ug
EUR 355

Monoclonal Functional BAFF-R (mouse) Antibody, mAb , Clone: 9B9

APR16043G 0.1mg
EUR 528
Description: A Monoclonal antibody against Human Functional BAFF-R (mouse), mAb . The antibodies are raised in Purified From Concentrated Hybridoma Tissue Culture Supernatant. and are from clone 9B9. This antibody is applicable in FC

Anti-ERK1/2 (E31R) Signal Transduction Monoclonal Antibody

ABP-MAB-ES003 100 ug Ask for price

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody

ABP-MAB-RT008 100 ug Ask for price

Anti-PON1 Biotinylated Antibody

A00516-Biotin 50ug/vial
EUR 294

Magic™ Anti-IBV Mab, Clone N53242

DCABY-4536 1 mg
EUR 754

Magic™ Anti-IBV Mab, Clone N23323

DCABY-4539 1 mg
EUR 754

Mouse Anti-Omethoate monoclonal antibody, clone OMO

HMABPY0001-100ug 100 ug
EUR 533

Mouse Anti-Omethoate monoclonal antibody, clone OMO

HMABPY0001-1mg 1 mg
EUR 1313

Mouse Anti-Metalaxyl monoclonal antibody, clone MA

HMABPY0003-100ug 100 ug
EUR 533

Mouse Anti-Metalaxyl monoclonal antibody, clone MA

HMABPY0003-1mg 1 mg
EUR 1313

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK1

HMABPY082-100ug 100 ug
EUR 533

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK1

HMABPY082-1mg 1 mg
EUR 1313

Mouse Anti-Ovalbumin monoclonal antibody, clone OVAD

HMABPY118D-100ug 100 ug
EUR 533

Mouse Anti-Ovalbumin monoclonal antibody, clone OVAD

HMABPY118D-1mg 1 mg
EUR 1313

Mouse Anti-PNA monoclonal antibody, clone PNAD

HMABPY119D-100ug 100 ug
EUR 533

Mouse Anti-PNA monoclonal antibody, clone PNAD

HMABPY119D-1mg 1 mg
EUR 1313

Mouse Anti-Casein monoclonal antibody, clone CSD

HMABPY120D-100ug 100 ug
EUR 533

Mouse Anti-Casein monoclonal antibody, clone CSD

HMABPY120D-1mg 1 mg
EUR 1313

Mouse Anti-Albendazole monoclonal antibody, clone ABZ2

CABT-L3042-100ug 100 ug
EUR 533

Mouse Anti-Albendazole monoclonal antibody, clone ABZ2

CABT-L3042-1mg 1 mg
EUR 1313

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK2

CABT-L3046-100ug 100 ug
EUR 533

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK2

CABT-L3046-1mg 1 mg
EUR 1313

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK3

CABT-L3047-100ug 100 ug
EUR 533

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK3

CABT-L3047-1mg 1 mg
EUR 1313

Mouse Anti-Cyproheptadine monoclonal antibody, clone CHD

CABT-L3052-100ug 100 ug
EUR 533

Mouse Anti-Cyproheptadine monoclonal antibody, clone CHD

CABT-L3052-1mg 1 mg
EUR 1313

Mouse Anti-Clonidine monoclonal antibody, clone CLD

CABT-L3053-100ug 100 ug
EUR 533

Mouse Anti-Clonidine monoclonal antibody, clone CLD

CABT-L3053-1mg 1 mg
EUR 1313

Mouse Anti-Diaveridine monoclonal antibody, clone DVD

CABT-L3055-100ug 100 ug
EUR 533

Mouse Anti-Diaveridine monoclonal antibody, clone DVD

CABT-L3055-1mg 1 mg
EUR 1313

Mouse Anti-Ormetoprim monoclonal antibody, clone OMP

CABT-L3056-100ug 100 ug
EUR 533

Mouse Anti-Ormetoprim monoclonal antibody, clone OMP

CABT-L3056-1mg 1 mg
EUR 1313

Mouse Anti-Clindamycin monoclonal antibody, clone CDM

CABT-L3057-100ug 100 ug
EUR 533

Mouse Anti-Clindamycin monoclonal antibody, clone CDM

CABT-L3057-1mg 1 mg
EUR 1313

Mouse Anti-Azithromycin monoclonal antibody, clone ATM

CABT-L3058-100ug 100 ug
EUR 533

Mouse Anti-Azithromycin monoclonal antibody, clone ATM

CABT-L3058-1mg 1 mg
EUR 1313

Mouse Anti-Roxithromycin monoclonal antibody, clone RTM

CABT-L3059-100ug 100 ug
EUR 533

Mouse Anti-Roxithromycin monoclonal antibody, clone RTM

CABT-L3059-1mg 1 mg
EUR 1313

Mouse Anti-Clarithromycin monoclonal antibody, clone CTM

CABT-L3060-100ug 100 ug
EUR 533

Mouse Anti-Clarithromycin monoclonal antibody, clone CTM

CABT-L3060-1mg 1 mg
EUR 1313

Mouse Anti-Spiramycin monoclonal antibody, clone SPM

CABT-L3062-100ug 100 ug
EUR 533

Mouse Anti-Spiramycin monoclonal antibody, clone SPM

CABT-L3062-1mg 1 mg
EUR 1313

Structural and functional characterization of C0021158, a high-affinity monoclonal antibody that inhibits Arginase 2 function via a novel non-competitive mechanism of action

  • Arginase 2 (ARG2) is a binuclear manganese metalloenzyme that catalyzes the hydrolysis of L-arginine. The dysregulated expression of ARG2 within specific tumor microenvironments generates an immunosuppressive niche that effectively renders the tumor ‘invisible’ to the host’s immune system.
  • Increased ARG2 expression leads to a concomitant depletion of local L-arginine levels, which in turn leads to suppression of anti-tumor T-cell-mediated immune responses. Here we describe the isolation and characterization of a high affinity antibody (C0021158) that inhibits ARG2 enzymatic function completely, effectively restoring T-cell proliferation in vitro. Enzyme kinetic studies confirmed that C0021158 exhibits a noncompetitive mechanism of action, inhibiting ARG2 independently of L-arginine concentrations.
  • To elucidate C0021158’s inhibitory mechanism at a structural level, the co-crystal structure of the Fab in complex with trimeric ARG2 was solved. C0021158’s epitope was consequently mapped to an area some distance from the enzyme’s substrate binding cleft, indicating an allosteric mechanism was being employed. Following C0021158 binding, distinct regions of ARG2 undergo major conformational changes.
  • Notably, the backbone structure of a surface-exposed loop is completely rearranged, leading to the formation of a new short helix structure at the Fab-ARG2 interface. Moreover, this large-scale structural remodeling at ARG2’s epitope translates into more subtle changes within the enzyme’s active site.
  • An arginine residue at position 39 is reoriented inwards, sterically impeding the binding of L-arginine. Arg39 is also predicted to alter the pKA of a key catalytic histidine residue at position 160, further attenuating ARG2’s enzymatic function.
  • In silicomolecular docking simulations predict that L-arginine is unable to bind effectively when antibody is bound, a prediction supported by isothermal calorimetry experiments using an L-arginine mimetic.

    Specifically, targeting ARG2 in the tumor microenvironment through the application of C0021158, potentially in combination with standard chemotherapy regimens or alternate immunotherapies, represents a potential new strategy to target immune cold tumors.

Development of a physiologically-based pharmacokinetic model for ocular disposition of monoclonal antibodies in rabbits

  • Development of protein therapeutics for ocular disorders, particularly age-related macular degeneration (AMD), is a highly competitive and expanding therapeutic area.
  • However, the application of a predictive and translatable ocular PK model to better understand ocular disposition of protein therapeutics, such as a physiologically-based pharmacokinetic (PBPK) model, is missing from the literature.
  • Here, we present an expansion of an antibody platform PBPK model towards rabbit and incorporate a novel anatomical and physiologically relevant ocular component.
  • Parameters describing all tissues, flows, and binding events were obtained from existing literature and fixed a priori. First, translation of the platform PBPK model to rabbit was confirmed by evaluating the model’s ability to predict plasma PK of a systemically administered exogenous antibody.
  • Then, the PBPK model with the new ocular component was validated by estimation of serum and ocular (i.e. aqueous humor, retina, and vitreous humor) PK of two intravitreally administered monoclonal antibodies.
  • We show that the proposed PBPK model is capable of accurately (i.e. within twofold) predicting ocular exposure of antibody-based drugs. The proposed PBPK model can be used for preclinical-to-clinical translation of antibodies developed for ocular disorders, and assessment of ocular toxicity for systemically administered antibody-based therapeutics.

Development of High Resolution DNA Melting Analysis for Simultaneous Detection of Potato Mop-Top Virus and Its Vector, Spongospora subterranea, in Soil

 In this study, a set of duplex reverse transcription (RT)-PCR-mediated high resolution DNA melting (HRM) analyses for simultaneous detection of potato mop-virus (PMTV) and its protist vector, Spongospora subterranea f.sp. subterranea (Sss), was developed.

The infestation of soil by PMTV was detected by using a tobacco-based baiting system. Total RNA extracted from the soil led to successful RT-PCR gel-electrophoresis detection of both PMTV and Sss. To facilitate more efficient detection, newly designed primer pairs for PMTV RNA species (i.e., RNA-Rep, -CP, and -TGB) were analyzed together with the existing Sss primers using real-time RT-PCR.

The resulting amplicons exhibited melting profiles that could be readily differentiated. Under duplex RT-PCR format, all PMTV and Sss primer combinations led to successful detection of respective PMTV RNA species and Sss in the samples by high resolution DNA melting (HRM) analyses. When the duplex HRM assay was applied to soil samples collected from six fields at four different sites in New Brunswick, Canada, positive detection of PMTV and/or Sss was found in 63-100% samples collected from fields in which PMTV-infected tubers had been observed.

In contrast, the samples from fields where neither PMTV- nor Sss-infected tubers had been observed resulted in negative detection by the assay. Bait tobacco bioassay for PMTV and Sss produced similar results. Between 63%-83% and 100% of the soil samples collected from PMTV-infested fields led to PMTV and Sss infections in the bait tobacco plants, respectively; whereas no PMTV or Sss infected plants were obtained from soil samples collected from PMTV/Sss-free fields.

Mouse Paraoxonase 1 (PON1) ELISA Kit

RDR-PON1-Mu-96Tests 96 Tests
EUR 709

Anti-PON1 Antibody (monoclonal, 9D3)

M00516 100ug/vial
EUR 334

Anti-PON1 Antibody (monoclonal, 6B1)

M00516-1 100ug/vial
EUR 334

Anti-PON1 Antibody (monoclonal, 11H2)

M00516-2 100ug/vial
EUR 294

Anti-PON1 Clone KRJ1 (100 µg)

0801012 100 µg
EUR 284
Description: Please contact Gentaur in order to receive the datasheet of the product.

Anti-PON1 Clone KRJ1 (1 mg)

0801095 1 mg
EUR 1689.04
Description: Please contact Gentaur in order to receive the datasheet of the product.

Rabbit Anti-Human PON1 Monoclonal Antibody

CABT-36017RH 100ug
EUR 840

anti-PON1

YF-PA24422 50 ul
EUR 334
Description: Mouse polyclonal to PON1

Mouse Monoclonal Anti-Mouse CD160 mAb, Biotin, (clone CNX46-3), (mouse IgG2bk)

MCD160-B 100 tests
EUR 469

Mouse Monoclonal Anti-Mouse CD160 mAb, FITC, (clone CNX46-3), (mouse IgG2bk)

MCD160-F 50 Tests
EUR 408

Mouse Monoclonal Anti-Mouse CD160 mAb, Purified, (clone CNX46-3), (mouse IgG2bk)

MCD160-M 100 ug
EUR 482

Mouse Monoclonal Anti-Mouse CD160 mAb, PE, (clone CNX46-3), (mouse IgG2bk)

MCD160-PE 50 tests
EUR 347

Human Paraoxonase 1 (PON1) ELISA Kit

RD-PON1-Hu-48Tests 48 Tests
EUR 478

Human Paraoxonase 1 (PON1) ELISA Kit

RD-PON1-Hu-96Tests 96 Tests
EUR 662

Rat Paraoxonase 1 (PON1) ELISA Kit

RD-PON1-Ra-48Tests 48 Tests
EUR 511

Rat Paraoxonase 1 (PON1) ELISA Kit

RD-PON1-Ra-96Tests 96 Tests
EUR 709

Human Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Hu-48T 48T
EUR 479
Description: A sandwich quantitative ELISA assay kit for detection of Human Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Hu-96T 96T
EUR 621
Description: A sandwich quantitative ELISA assay kit for detection of Human Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Ra-48T 48T
EUR 508
Description: A sandwich quantitative ELISA assay kit for detection of Rat Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Paraoxonase 1 (PON1) ELISA Kit

DLR-PON1-Ra-96T 96T
EUR 661
Description: A sandwich quantitative ELISA assay kit for detection of Rat Paraoxonase 1 (PON1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Paraoxonase 1 (PON1) ELISA Kit

RDR-PON1-Hu-48Tests 48 Tests
EUR 500

Human Paraoxonase 1 (PON1) ELISA Kit

RDR-PON1-Hu-96Tests 96 Tests
EUR 692

Rat Paraoxonase 1 (PON1) ELISA Kit

RDR-PON1-Ra-48Tests 48 Tests
EUR 534

Rat Paraoxonase 1 (PON1) ELISA Kit

RDR-PON1-Ra-96Tests 96 Tests
EUR 742

Anti-GFP (GF28R) Tag monoclonal antibody

ABP-MAB-GT007 100 ug Ask for price

PON1 recombinant monoclonal antibody

A5406 100ul X 3
EUR 595
Description: A recombinant monoclonal antibody from rabbit against human PON1 for WB, IHC,ELISA

Anti-?-Actin (BA3R) Loading Control Monoclonal Antibody

ABP-MAB-BL002 100 ug Ask for price

Anti-DYKDDDDK (FLAG) Tag (FG4R) Monoclonal Antibody

ABP-MAB-DT006 100 ug Ask for price

Anti-ERK1 (E32) Signal Transduction Monoclonal Antibody

ABP-MAB-ES001 100 ug Ask for price

Anti-ERK1 (E19) Signal Transduction Monoclonal Antibody

ABP-MAB-ES002 100 ug Ask for price

Anti-GAPDH (GA1R) Loading Control Monoclonal Antibody

ABP-MAB-GL001 100 ug Ask for price

Anti-GST Tag (GST.B6 / G2R) Monoclonal Antibody

ABP-MAB-GT003 100 ug Ask for price

Anti-His Tag (HIS.H8 / EH158) Monoclonal Antibody

ABP-MAB-HT001 100 ug Ask for price

Anti-HA Tag (HA.C5 / C10) Monoclonal Antibody

ABP-MAB-HT002 100 ug Ask for price

Anti-Myc Tag (Myc.A7 / M19) Monoclonal Antibody

ABP-MAB-MT005 100 ug Ask for price

Anti-V5 Tag (E10 / V4RR) Monoclonal Antibody

ABP-MAB-VT004 100 ug Ask for price

Monoclonal Functional BAFF-R (mouse) Antibody, mAb , Clone: 9B9

APR16043G 0.1mg
EUR 528
Description: A Monoclonal antibody against Human Functional BAFF-R (mouse), mAb . The antibodies are raised in Purified From Concentrated Hybridoma Tissue Culture Supernatant. and are from clone 9B9. This antibody is applicable in FC

Anti-PON1 Antibody

PA2253 100ug/vial
EUR 294

Anti-PON1 antibody

STJ25055 100 µl
EUR 277
Description: The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.

Anti-PON1 antibody

STJ25056 100 µl
EUR 277
Description: The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.

Anti-PON1 antibody

STJ29477 100 µl
EUR 277
Description: The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.

Anti-PON1 Antibody

A00516 100ug/vial
EUR 334

Anti-PON1 Antibody

A00516-1 100ug/vial
EUR 294

Anti-PON1 Antibody

A00516-3 100ug/vial
EUR 334

Anti-PON1 antibody

STJ98804 200 µl
EUR 197
Description: Rabbit polyclonal to PON1.

anti- PON1 antibody

FNab06638 100µg
EUR 505.25
Description: Antibody raised against PON1

Anti-PON1 antibody

PAab06638 100 ug
EUR 355

Anti-PON1 (2H7)

YF-MA14808 100 ug
EUR 363
Description: Mouse monoclonal to PON1

Anti-ERK1/2 (E31R) Signal Transduction Monoclonal Antibody

ABP-MAB-ES003 100 ug Ask for price

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody

ABP-MAB-RT008 100 ug Ask for price

Anti-PON1 Biotinylated Antibody

A00516-Biotin 50ug/vial
EUR 294

Magic™ Anti-IBV Mab, Clone N53242

DCABY-4536 1 mg
EUR 754

Magic™ Anti-IBV Mab, Clone N23323

DCABY-4539 1 mg
EUR 754

Mouse Anti-Omethoate monoclonal antibody, clone OMO

HMABPY0001-100ug 100 ug
EUR 533

Mouse Anti-Omethoate monoclonal antibody, clone OMO

HMABPY0001-1mg 1 mg
EUR 1313

Mouse Anti-Metalaxyl monoclonal antibody, clone MA

HMABPY0003-100ug 100 ug
EUR 533

Mouse Anti-Metalaxyl monoclonal antibody, clone MA

HMABPY0003-1mg 1 mg
EUR 1313

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK1

HMABPY082-100ug 100 ug
EUR 533

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK1

HMABPY082-1mg 1 mg
EUR 1313

Mouse Anti-Ovalbumin monoclonal antibody, clone OVAD

HMABPY118D-100ug 100 ug
EUR 533

Mouse Anti-Ovalbumin monoclonal antibody, clone OVAD

HMABPY118D-1mg 1 mg
EUR 1313

Mouse Anti-PNA monoclonal antibody, clone PNAD

HMABPY119D-100ug 100 ug
EUR 533

Mouse Anti-PNA monoclonal antibody, clone PNAD

HMABPY119D-1mg 1 mg
EUR 1313

Mouse Anti-Casein monoclonal antibody, clone CSD

HMABPY120D-100ug 100 ug
EUR 533

Mouse Anti-Casein monoclonal antibody, clone CSD

HMABPY120D-1mg 1 mg
EUR 1313

Mouse Anti-Sulfonamides monoclonal antibody, clone SF2

DMAB8840-100ug 100 ug
EUR 533

Mouse Anti-Sulfonamides monoclonal antibody, clone SF2

DMAB8840-1mg 1 mg
EUR 1313

Mouse Anti-Sulfonamides monoclonal antibody, clone SF1

DMAB88404M-100ug 100 ug
EUR 533

Mouse Anti-Sulfonamides monoclonal antibody, clone SF1

DMAB88404M-1mg 1 mg
EUR 1313

Mouse Anti-TGEV monoclonal antibody, clone 322.65

DMABT-Z60015 1 mg
EUR 819

Mouse Anti-Albendazole monoclonal antibody, clone ABZ2

CABT-L3042-100ug 100 ug
EUR 533

Mouse Anti-Albendazole monoclonal antibody, clone ABZ2

CABT-L3042-1mg 1 mg
EUR 1313

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK2

CABT-L3046-100ug 100 ug
EUR 533

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK2

CABT-L3046-1mg 1 mg
EUR 1313

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK3

CABT-L3047-100ug 100 ug
EUR 533

Mouse Anti-Fluoroquinolones monoclonal antibody, clone FK3

CABT-L3047-1mg 1 mg
EUR 1313

Mouse Anti-Cyproheptadine monoclonal antibody, clone CHD

CABT-L3052-100ug 100 ug
EUR 533

Mouse Anti-Cyproheptadine monoclonal antibody, clone CHD

CABT-L3052-1mg 1 mg
EUR 1313

Mouse Anti-Clonidine monoclonal antibody, clone CLD

CABT-L3053-100ug 100 ug
EUR 533

Mouse Anti-Clonidine monoclonal antibody, clone CLD

CABT-L3053-1mg 1 mg
EUR 1313

Mouse Anti-Diaveridine monoclonal antibody, clone DVD

CABT-L3055-100ug 100 ug
EUR 533

Mouse Anti-Diaveridine monoclonal antibody, clone DVD

CABT-L3055-1mg 1 mg
EUR 1313

Mouse Anti-Ormetoprim monoclonal antibody, clone OMP

CABT-L3056-100ug 100 ug
EUR 533

Mouse Anti-Ormetoprim monoclonal antibody, clone OMP

CABT-L3056-1mg 1 mg
EUR 1313

Mouse Anti-Clindamycin monoclonal antibody, clone CDM

CABT-L3057-100ug 100 ug
EUR 533

Mouse Anti-Clindamycin monoclonal antibody, clone CDM

CABT-L3057-1mg 1 mg
EUR 1313

Mouse Anti-Azithromycin monoclonal antibody, clone ATM

CABT-L3058-100ug 100 ug
EUR 533

Mouse Anti-Azithromycin monoclonal antibody, clone ATM

CABT-L3058-1mg 1 mg
EUR 1313

Mouse Anti-Roxithromycin monoclonal antibody, clone RTM

CABT-L3059-100ug 100 ug
EUR 533

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